Article Published In Vol. 01, Issue 02 (2021)

Measuring the Growth of Lactobacillus fermentum and Bifidobacterium infantis in the presence of N-Acetyl L-Cysteine Hydrochloric Acid

Author : Harsh Pratap Singh, Pradip Kumar Sharma Mahesh Kumar and Parul Sharma

Aim: To study the growth of probiotic microorganisms including Lactobacillus fermentum and Bifidobacterium infantis in presence of N-Acetyl L-Cysteine Hydrochloric Acid (NACHCl)

Settings and Design:  Lactobacillus fermentum and Bifidobacterium infantis growth in the presence of N-Acetyl L-Cysteine Hydrochloric Acid were studied by using MRS broth without any additional supplement as a control.

Material and Methods: Lactobacillus fermentum and Bifidobacterium infantis isolated from fecal samples of infants and adults were collected. Positive culture of Lactobacillus fermentum and Bifidobacterium infantis were collected from NCDC NDRI Karnal. Different concentrations of NACHCl including 0.2, 0.4, 0.6, 0.8 and 1.0 g/L were used in this study.

Results: The results were observed up to 16 hours of incubation where in 1.0 g/L i.e. the highest concentration give rise to the optical density of 1.577 for Bifidobacterium infantis while for Lactobacillus fermentum1.792 when measured at 600nm, however controls of both the cultures reached 1.136 and 1.108 respectively.

Conclusion:  The application of N-Acetyl L-Cysteine Hydrochloric Acid certainly supports the growth of probiotic flora to a very good extent and can act as a potential prebiotic.

Keywords: Lactobacillus fermentum, Bifidobacterium infantis, N-Acetyl L-Cysteine HCl and MRS broth