Article Published In Vol. 05, Issue 01 (2025)

Design, Synthesis and Pharmacological evaluation of Novel Pyrrolidine analogues as potent Antibacterial agents: Experimental and Molecular docking approach

Author : Ojas Patel, M.R. Patel, R. Vasava

Abstract

Background: Pyrrolidine and its derivatives are extremely important in medicinal chemistry due to their enormous variety and possible pharmacological activity. Synthesis of various pyrrolidine derivatives of biological interest, which have been reported in a wide spectrum of pharmacological activities

Material and methods: According to a synthetic approach, a number of acetyl pyrrolidine 2-carboxamide compounds have been synthesized. Mass spectrum analysis, infrared, and proton nuclear magnetic resonance were used to analyze the synthesized compounds. These substances were examined for their antibacterial properties against a range of bacterial strains in order to determine their biological activity. Additionally, the molecular docking investigation confirmed the experimental conclusions.

Results: A variety of new pyrrolidine-2-carboxamide derivatives have been synthesized with good yield, successfully characterized by TLC, IR, NMR and Mass Spectra and evaluated for their antibacterial activity. Compound 1 with the pyrrolidine group (-7.4 kcal/mol) shown a strong binding affinity with compared to Streptomycin (-7.1 kcal/mol) with 3uzu binding protein in the docking.

Conclusion: All of the newly synthesized compounds showed moderate to good antibacterial activity against both Gram positive and Gram negative strains. When compared to Streptomycin, compounds with the pyrrolidine and piperazine groups exhibit the largest zone of inhibitions against three bacterial strains.

Keywords: Pyrrolidine, Antibacterial, Antimicrobial, Molecular docking, Streptomycin.