BIOLOGICAL DEGRADATION OF PHARMACEUTICAL PRODUCT NORFLOXACIN BY SELECTED BACTERIAL ISOLATES
Pages : 62-68Download PDF
Abstract
Aim: Pharmaceutical pollutants that stay in the environment for a long time, like the antibiotic norfloxacin, cause big health and environmental problems that need effective bioremediation strategies. The aim of the study is to see degradation of Norfloxacin by selected bacterial isolates.
Method: This research looked into how bacteria from dirt samples taken from clean and nearby pharmaceutical industries break down norfloxacin. The pH ranged from 6.50 to 8.30, and the temperature ranged from 19°C to 40°C. Ten types of bacteria were found through enrichment and isolation. Seven of them were able to survive norfloxacin amounts of up to 500 ppm.
Result: The isolates NFX-5a, NFX-7a, and NFX-8a showed the most tolerance and growth. After 72 hours, NFX-7a had the highest degradation rate (80.63%), followed by NFX-5a (61.91%) and consortium BC (NFX-7a + NFX-8a) (50.24%). Morphological and biochemical studies showed a mixture of Gram-positive and Gram-negative bacteria that were mostly rod-shaped and had a range of metabolic skills, such as catalase activity and sugar fermentation. The results show that NFX-7a and other isolates may be able to break down norfloxacin bioremediation, giving us information about how well they break it down and how metabolically flexible they are.
Conclusion: These results show that bacterial isolates have a lot of potential to clean up pharmaceutical pollution. They also open the door to more research into how their enzymes work and how they can be used in real life to clean up the environment.
Keywords: Biological degradation, Norfloxacin, Environmental contamination, Microbial degradation